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Introduction

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In the basic research of life sciences and medicine, antibodies are more or less needed. The application scenarios of antibodies are different, there are many markers, and for the same marker, there are often multiple antibodies to choose from in the market. We need to select the one we need from millions or tens of millions of antibodies. Therefore, CiteAb was created.

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CiteAb (www.citeab.com) was founded in 2014 led by Dr. Andrew Chalmers from the University of Bath, UK. It claims to be the world’s largest antibody search engine, containing nearly five million antibodies from 204 antibody suppliers, summarizing over two million antibody citation data, and the website continues to update the latest data. By inputting keywords (such as: CD3) and clicking search, all matching antibodies can be displayed. The platform currently includes 6.99 million antibodies from 335 suppliers, with citation numbers exceeding 4.24 million. The feature of this website is that the search results are sorted according to the number of citations the antibody has been referenced in literature. Generally speaking, products with more citations are more likely to have better quality assurance. The CiteAb website interface is simple and clear. In addition to searching for antibodies, it can also query biochemical reagents, experimental methods, reagent kits and proteins.

Register account

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Register at https://www.citeab.com/accounts/sign_up. Here we need to use a .edu college email address. Only after registering can we use Citeab’s search engine services.

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For the sake of convenient registration, this tutorial will mainly use American edu email addresses. As shown in the picture below:

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Create your free CiteAb account today.

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Register for an account to maximise the benefits of our reagent search engine.

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Please use your institutional email address, input your organization’s email address, which is usually an academic email address. A university edu email address is an academic email address.

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Country, Institution or organisation name, Usage: Academia

Account verification

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After filling in the information and clicking confirm, an email will be received in the edu mailbox to confirm the CiteAb account email.Here is my translation of the original Chinese text to English while following the given guidelines:

Instructions for confirmation

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“You can confirm your account email and start using CiteAb by following the link below:” “You can confirm your account email and start using CiteAb by following the link below.”

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Thank you for signing up to CiteAb,Please check your inbox.

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We need to confirm your email address. A message with a confirmation link has been sent to czane13sb@stu.xx.edu.

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We need to confirm your email address. A message with a confirmation link has been sent to czane13sb@stu.xx.edu.

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Please click the link to activate your account and gain full access to CiteAb. Please click the link to activate your account and gain full access to CiteAb.

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Your link should arrive within a few minutes. If you haven’t received an email please check your spam or request that we resend your confirmation link. 您的链接应该会在几分钟内到达。如果您没有收到电子邮件,请检查您的垃圾邮件或要求我们重新发送您的确认链接。

Registration successful.

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After clicking authentication, we were prompted: “Your email address has been successfully confirmed.” At this point, our CiteAb account has been successfully activated and ready for use.

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Usage

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As an example, we use the commonly used internal control β-actin to perform a CiteAb search. In the search bar, enter the query antibody name “β-actin” (only supports English queries), and in the directory after the search bar select “Antibodies”, click “Search” to obtain the following results.

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It can be seen that the page simply records some basic information about the antibody, including the item number in red font and the antibody company, as well as the antibody host type (source), applicability, species reactivity and clone type (monoclonal or polyclonal), beside it is the number of times the antibody was cited, the search results are arranged from high to low according to the number of times the antibody was cited, the more times it was cited, the more reliable the antibody is relatively.

Here is the translation:

In addition, the left column can search for antibodies according to the primary and secondary antibody type, clone type, whether recombinant, host type, verification method, experimental application type, species reactivity, supplier and other multiple directories for classification, so as to quickly find the antibody suitable for one’s own experiment.

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After randomly clicking into an antibody, there will be more detailed supplemental information, and most importantly is the literature list of applications for that antibody within, it can be said the reliability is extremely high.